RA or Scientist, Next-generation Sequencing
convergentresearch.org
Watertown, MA, USA
Posted on Thursday, August 10, 2023
Cultivarium is a Convergent Research focused research organization with a mission to accelerate the adoption of new organisms for biotechnology. We are looking for experts who can contribute both individually and as part of a team to create tangible solutions for a ‘moonshot’ project. This is a great opportunity to be part of a close-knit, driven team of scientists and engineers that are building technological infrastructure for non-model microorganisms, to open new avenues for science, technology, medicine and agriculture.We are looking for a next-generation sequencing specialist to join the molecular biology team. You will contribute to construction, development, and application of sequencing libraries and genetic tools for studying and engineering non-model microbes. We’re searching for a candidate who is proficient in next-generation sequencing library preparation and motivated to expand their molecular biology and microbiology skill set. You will work closely with molecular biologists, microbiologists, automation and software engineers. This is a full-time experimental role and must be on-site.
Responsibilities
- Design, plan and execute NGS-based assays in coordination with interdisciplinary science teams, including loading and running libraries on Illumina and Oxford Nanopore instruments
- Perform nucleic acid isolation or preparation for genome or transcriptomes from diverse bacteria, yeast, fungi, or viruses
- Maintain high standards for sequencing quality and throughput by rigorously performing in-house QC checks (qPCR, Qubit, and gel electrophoresis) and remaining current with industrial best practices
- Write and maintain SOPs for NGS assays, maintain sequencer uptime and manage reagent inventory.
- Demonstrate a commitment to diversity, inclusion, and cultural awareness through actions, interactions, and communications with others
- BS, MS, or PhD in biomedical engineering, genetics, microbiology or related field that relies on next-generation sequencing applications
- High proficiency with nucleic acid preparation from microbial and/or eukaryotic sources, Mechanical or enzymatic-based shearing
- High proficiency with molecular biology techniques, including ligation, PCR, and other enzymatic-based nucleic acid manipulations
- Quantification of nucleic acids with spectrophotometric or fluorescence-based assays
- Strong analytical and quantitative approach to laboratory science
- Proven track record of custom library-preparation development for next-generation sequencing
- Authorized to work in the U.S. without sponsorship
- Prior experience with diverse next-generation sequencing technologies such as Pacific Biosciences, Oxford Nanopore, and/or Ion Torrent
- Experience with laboratory liquid handling automation for sequencing library preparation
- Familiarity and proficiency with programming and development of custom protocols
- Advanced degree in engineering or biological sciences and/or industrial experience in related fields
- An opportunity to change the world and work with some of the smartest and the most talented experts from different fields
- Excellent medical, dental, and vision insurance through a PPO plan; parental leave
- Generous time off + paid holidays
- Wellness allowance for fitness and wellness activities